Varied response of detoxification enzyme activities against lethal and sublethal exposures of phosphine in Tribolium castaneum populations

Resistance to phosphine fumigation in stored insects continues be challenging managing these pests worldwide. The role of detoxification enzymes metabolizing is yet explored fully. dose-response mortality against was assessed this study from six field populations Tribolium castaneum (Herbst) collected across India during 2021–23 at the Division Entomology, ICAR-Indian Agricultural Research Institute, New Delhi. Further, strength Glutathione-S-Transferase (GST) and Cytochrome P450 monooxygenase (CYPs) estimated sublethal (LC30) median lethal concentration (LC50). Dose-response probit assay found that LC30 LC50 values ranged 0.018 0.363 0.038 1.277 mg/L, respectively. Our result showed MZ (Mirzapur) KA (Kailashahar) were high least resistant compared susceptible reference LS (lab population). magnitude GST CYPs activities more elevated than populations. enhancement activity eight nine-fold two three-fold concentrations, Correlation analysis revealed a significant positive association between (r=0.94, P<0.01; r=0.98, P<0.001) r=0.97, both findings implied escalated with increased resistance exposure T. castaneum.